| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nguyen, Olivia B (10)
|
MRSSC |
34.91 |
35.23 |
0.32 |
| 2 |
Vargas, Kaylee M (10)
|
MRTVSC |
35.94 |
35.39 |
-0.55 |
| 3 |
Lisica, Violet E (10)
|
MRTVSC |
42.74 |
44.00 |
1.26 |
| 4 |
Wiedersum, Sadie K (10)
|
MRTVSC |
38.87 |
44.34 |
5.47 |
| 5 |
Fletcher, Reilly D (10)
|
MRFA |
48.10 |
44.81 |
-3.29 |
| 6 |
Moran, Olivia K (10)
|
MRFA |
55.81 |
48.75 |
-7.06 |
| 7 |
Villagracia, Madison E (9)
|
MRTVSC |
56.54 |
49.59 |
-6.95 |
| 8 |
Surendra, Azzurra P (9)
|
MRTVSC |
53.59 |
50.40 |
-3.19 |
| 9 |
Surendra, Bianca P (9)
|
MRTVSC |
49.92 |
52.25 |
2.33 |
| 10 |
Robertson, Quinn E (10)
|
MRHYB |
57.64 |
54.07 |
-3.57 |
| 11 |
Merenkova, Anastasia (10)
|
MRTVSC |
54.85 |
58.03 |
3.18 |
| 12 |
Katakofsky, Lena N (9)
|
MRFA |
1:11.39 |
1:04.29 |
-7.10 |